Translational_Unit

Part:BBa_K322210:Experience

Designed by: Geraldine Avila   Group: iGEM10_Edinburgh   (2010-09-25)

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Applications of BBa_K322210

Characterisation of the activity of the cat BioBrick:


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Cell lines containing cat in pSB1A2 (red boxes) grew on both ampicillin (100mg/ml) chloramphenicol (40mg/ml) whereas cell lines containing a pSB1A2 control (Edinbrick, blue boxes) only grew on ampicillin (100mg/ml). This experiment confirms that cat is effective as a negative selection marker within the BRIDGE protocol.

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